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Can f 1 ELISA kit 狗過敏原Can f1檢測試劑盒

產品分類 > 過敏疾病檢測原料 > Can f 1 ELISA kit 狗過敏原Can f1檢測試劑盒

Can f 1 ELISA kit 狗過敏原Can f1檢測試劑盒

產品貨號：EL-CF1

促銷：￥12720.00

價格：￥12720.00

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產品規格：

kit

供應商：

美國Indoor

詳細介紹

ELISA kit for measuring Canis familiaris allergen, Can f 1.
Antibodies:	10D4/pAb
Sensitivity:	0.5ng/ml
Limit of Detection:	0.5ng/ml
Assay Performance:	Intra-assay CV% of 6.0; Inter-assay CV% of 9.5
Contents:	Monoclonal antibody 10D4, Universal Allergen Standard, Polyclonal Rabbit anti-Can f 1; sufficient reagents for 10 microtiter plates.
Storage:	The ELISA kit should be stored at 4°C.
Not Provided:	50mM carbonate/bicarbonate buffer, pH 9.6 Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20 (PBS-T) Bovine serum albumin Peroxidase conjugated Goat anti. Rabbit IgG 1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2
Notes:
Product Resources:	EL-CF1 Certificate of Analysis
For research and commercial use in vitro: not for human in vivo or therapeutic use.
	Certificate of Analysis
Monoclonal Antibody:	10D4 (clone 10D4 C4 A10)
Vial:	Red Cap; 100μl
Immunogen:	Can f 1
Isotype:	Mouse IgG2ak
Specificity:	Binds to an epitope on dog Canis familiarisallergen, Can f 1.
Purification:	Produced in ascites and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
Concentration:	1mg/ml in phosphate buffered saline, pH 7.4. Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.

	Certificate of Analysis
Antibody:	Rabbit polyclonal antiserum
Immunogen:	Dog fur extract
Vial:	Brown Cap; 100μl
Specificity:	The antiserum contains IgG antibodies to dog Canis familiaris allergen, Can f 1, as well as IgG antibodies to other dog allergens.
Activity:	Titrated for use in ELISA at 1/1000 dilution. Prepared in 1%BSA/ 50%glycerol/ PBS, pH 7.4., 0.22μm filtered, preservative free.

	Certificate of Analysis
Allergen Standard:	Universal Allergen Standard
Vial:	White Cap; 400μl
Composition:	A formulation of eight purified natural allergens prepared in 1% BSA/50% glycerol/PBS, pH 7.4.
Concentration/Calibration:
Universal Allergen Standard	Protein Measurement	Concentration (ng/ml)
Der p 1	Amino-acid analysis	2500
Der f 1	Amino-acid analysis	2500
Der p 2	Amino-acid analysis	1000
Fel d 1	Amino-acid analysis	1000
Can f 1	Amino-acid analysis	2500
Rat n 1	Amino-acid analysis	1000
Mus m 1	Amino-acid analysis	250
Bla g 2	Amino-acid analysis	2500
References:
1. Larsen JN, Ford A, Gjesing B, et al. The collaborative study of the international standard of dog, Canis domesticus, hair/dander extract. J Allergy Clin Immunol 1988; 82:318-330.
2. DeGroot H, Goei K, Van Sicter P, et al. Affinity purification of a major and minor allergen from dog extract: Serologic activity of affinity-purified Can f 1 and Can f 1-depleted extract. J Allergy Clin Immunol 1991; 87:1056-61.
3. Schou C, Hansen G, Linter T, et al. Assay for the major dog allergen, Can f 1 investigation of house dust samples and commercial dog extracts. J Allergy Clin Immunol 1992; 88:847-53.
4. Ingram JM, Sporik R, Rose G, Honsiger R, Chapman MD, Platts-Mills TAE. Quantitative assessment of exposure to dog (Can f 1) and cat (Fel d 1) allergens: Relationship to sensitization and asthma among children living in Los Alamos, New Mexico. J Allergy Clin Immunol 1995; 96:449-456.
5. Sporik RB, Ingram JM, Price W, Sussman JH, Honsiger RW, Platts-Mills TAE. Association of asthma with serum IgE and skin test reactivity to allergens among children living at high altitude: Tickling the dragon’s breath. Amer J Resp Crit Care Med 1995;151:1388-1392.
(For complete reference list see EL-CF1 Certificate of Analysis)