Antibodies:

6A8/4C1

Sensitivity:

0.5ng/ml

Limit of Detection:

2ng/ml

Assay Performance:

Intra-assay CV% of 7.1; Inter-assay CV% of 15

Contents:

Monoclonal antibody 6A8, Universal Allergen Standard,   Biotinylated antibody 4C1; sufficient reagents for 10 plates.

Storage:

Store at 4°C.

Not Provided:

50mM Carbonate/Bicarbonate buffer, pH 9.6
  Phosphate Buffered Saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
  Bovine Serum Albumin
  Streptavidin-Peroxidase
  1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:

Product Resources:

EL-DF1 Certificate of Analysis

For research and commercial use in vitro: not for   human in vivo or therapeutic use.

Certificate of Analysis

Monoclonal Antibody:

6A8 (clone 6A8 B10 D12)

Vial:

Red Cap; 100μl

Immunogen:

Der f 1

Isotype:

Mouse IgG1

Specificity:

Binds to a species specific epitope present on   Dermatophagoides farinae allergen, Der f 1.

Purification:

Produced in tissue culture and purified by affinity   chromatography using Protein A. Single heavy and light chain bands on   SDS-PAGE.

Concentration:

1mg/ml in phosphate buffered saline, pH 7.4. Based on   A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.

Certificate of Analysis

Monoclonal Antibody:

4C1 (clone 4C1 B8 3F8)

Vial:

Brown Cap; 100μl

Immunogen:

Der f 1

Isotype:

Mouse IgG1

Specificity:

Binds to a common epitope present on mite   Dermatophagoides Group 1 allergens (Der f 1, Der p 1, Der m 1, Eur m 1).

Purification:

Produced in ascites and purified by affinity   chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.

Biotinylation:

Biotinylated and titrated for use in ELISA at 1/1000   dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered,   preservative free.

Certificate of Analysis

Allergen Standard:

Universal Allergen Standard

Vial:

White Cap; 400μl

Composition:

A formulation of eight purified natural allergens   prepared in 1% BSA/50% glycerol/PBS, pH 7.4

Concentration/Calibration:

Universal Allergen Standard

Protein Measurement

Concentration (ng/ml)

Der p 1

Amino-acid analysis

2500

Der f 1

Amino-acid analysis

2500

Der p 2

Amino-acid analysis

1000

Fel d 1

Amino-acid analysis

1000

Can f 1

Amino-acid analysis

2500

Rat n 1

Amino-acid analysis

1000

Mus m 1

Amino-acid analysis

250

Bla g 2

Amino-acid analysis

2500

References:

1.   Chapman, M.D., Sutherland, W.M., and   Platts-Mills, T.A.E. (1984).  Recognition of two Dermatophagoides   pteronyssinus specific epitopes on Antigen P1 using  monoclonal   antibodies:  Binding to each epitope can be inhibited be sera from mite   allergic patients.  J. Immunol. 133:2488-2495.

2.  Ford, A.W., Rawle, F.C., Lind, P., Spieksma,   F.Th.M., Lowenstein, H. and Platts-Mills, T.A.E. (1985). Standardization of   Dermatophagoides pteronyssinus: Assessment of potency and allergen content in   ten coded extracts. Int. Archs. Allergy Appl. Immunol. 76:58-68.

3.  Heymann, P.W., Chapman, M.D. and Platts-Mills,   T.A.E. (1986). Antigen Der f 1 from the dust mite Dermatophagoides farinae:   Structural comparison with Der p 1 from D. pteronyssinus and epitope   specificity of murine IgG and human IgE antibodies. J. Immunol.   137:2841-2847.

4.   Chapman, M.D., Heyman, P.W., Wilkins,   S.R., Brown, M.J., and Platts-Mills, T.A.E. (1987). Monoclonal immunoassays   for major dust mite (Dermatophagoides) allergens, Der p 1 and Der f 1, and   quantitative analysis of the allergen content of mite and house dust   extracts. J. Allergy Clin. Immunol. 80:184-194.

5.  Platts-Mills, T.A.E. and Chapman, M.D. (1987).   Dust mites: Immunology, allergic disease and environmental control. J.   Allergy Clin. Immunol. 80:755-775

6.  Chapman, M.D., Heymann, P.W., and   Platts-Mills, T.A.E. (1987). Epitope mapping of two major inhalant allergens,   Der p 1 and Der f 1, from mites of the genus Dermatophagoides. J. Immunol.   139: 1479-1484.

7.  Chapman, M.D. (1988). Allergen specific   monoclonal antibodies: New tools for the management of allerigic disease.   Allergy 43 (5):7-14.

8.  Luczynska, C.M., Arruda, L.K., Platts-Mills,   T.A.E., Miller, J.D., Lopez, M., and Chapman, M.D. (1989). A two-site   monoclonal antibody ELISA for the quantification of the major   Dermatophagoides spp. allergens, Der p 1 and Der f 1. J. Immunol. Meths.   118:227-235.

9.  Platts-Mills T.A.E., Thomas W.R., Aalberse   R.C., Vervloet D. and Chapman M.D. (1992). Dust mite allergens and asthma:   Report of a second international workshop. J Allergy Clin Immunol   89:1046-1060.

10.  Platts-Mills T.A.E., Vervloet D., Thomas   W.R., Aalberse R.C., Chapman M.D. Indoor Allergens and Asthma: Report of the   third international workshop. J Allergy Clin Immunol, 1997; 100:S1-S24.

(For complete reference list see EL-DF1 Certificate of   Analysis)