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Bet v 1 ELISA kit 白樺過敏原Bet v 1檢測試劑盒

產品分類 > 過敏疾病檢測原料 > Bet v 1 ELISA kit 白樺過敏原Bet v 1檢測試劑盒

Bet v 1 ELISA kit 白樺過敏原Bet v 1檢測試劑盒

產品貨號：EL-BV1

促銷：￥12720.00

價格：￥12720.00

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kit

供應商：

美國Indoor

詳細介紹

ELISA kit for measuring Betula verrucosa allergen, Bet v 1.
Antibodies:		4B10/2E10
Sensitivity:		0.2ng/ml
Limit of Detection:		3.1ng/ml
Assay Performance:		Inquire
Contents:		Monoclonal antibody 4B10, Bet v 1 Standard, Biotinylated antibody 2E10; sufficient reagents for 10 plates.
Storage:		Store at 4°C.
Not Provided:		50mM Carbonate/Bicarbonate buffer, pH 9.6 Phosphate Buffered Saline, pH 7.4, containing 0.05% Tween 20 (PBS-T) Bovine Serum Albumin Streptavidin-Peroxidase 1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2
Notes:
Product Resources:		EL-BV1 Certificate of Analysis Bet v 1 Standard Letter - October 2017
For research and commercial use in vitro: not for human in vivo or therapeutic use.
		Certificate of Analysis
Monoclonal Antibody:		4B10 (clone 4B10)
Vial:		Red Cap; 100μl
Immunogen:		Bet v 1
Isotype:		Mouse IgG1
Specificity:		Binds to multiple isoforms of Betula verrucosa allergen, Bet v 1.
Purification:		Protein A affinity chromatography
Concentration:		1mg/ml in phosphate buffered saline, pH 7.4. Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.

		Certificate of Analysis
Monoclonal Antibody:		2E10 (clone 2E10 G6 G7)
Vial:		Brown Cap; 100μl
Immunogen:		Bet v 1
Isotype:		Mouse IgG1
Specificity:		Binds to a specific epitope on Betula verrucosa allergen, Bet v 1.
Purification:		Enrichment and concentration using 50% saturated ammonium sulphate precipitation followed by purification using protein A affinity chromatography.
Biotinylation:		Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free.

		Certificate of Analysis
Allergen Standard:		rBet v 1a
Vial:		White Cap; 400μl
Composition:		Recombinant Bet v 1a prepared in 1% BSA/50 glycerol/PBS, 0.22μm filtered, preservative free, pH 7.4.
Concentration:		1000ng/ml Bet v 1
Calibration:		The rBet v 1a was produced from Pichia and purified by a multi-step chromatography procedure. Validity of the rBet v 1 standard was confirmed by comparison with the European Pharmacopoeia reference CRS (Y0001565), recombinant major allergen rBet v 1 containing 10.25μg of rBet v 1 per vial.
References:
1. Breiteneder H, Pettenburger K, Bito A, et al. The gene coding for themajor birch pollen allergen Bet v 1, is highly homologous to a peadisease resistance response gene. EMBO J. 1989;8:1935-8. 2. Hoffmann-Sommergruber K, Susani M, Ferreira F, et al. High-levelexpression and purification of the major birch pollen allergen, Bet v 1.Protein Expr Purif. 1997;9:33-9. 3. Godnic-Cvar J, Susani M, Breiteneder H, et al. Recombinant Bet v 1, themajor birch pollen allergen, induces hypersensitivity reactions equal tothose induced by natural Bet v 1 in the airways of patients allergic to treepollen. J Allergy Clin Immu nol. 1997;99:354-9. 4. Schappi GF, Monn C, Wuthrich B, Wanner HU. Direct determination ofallergens in ambient aerosols: methodological aspects. Int Arch AllergyImmunol. 1996;110:364-70. 5. Schappi GF, Suphioglu C, Taylor PE, Knox RB. Concentrations of themajor birch tree allergen Bet v 1 in pollen and respirable fine particles inthe atmosphere. J Allergy Clin Immunol. 1997;100:656-61. 6. S. Vieths, D. Barber, M. Chapman, et al. Establishment of recombinantmajor allergens Bet v 1 and Phl p 5a as Ph. Eur. Reference standardsand validation of ELISA methods for their measurement. Pharmeuropa Bio&SN October 2012;118-13.
(For complete reference list see EL-BV1 Certificate of Analysis)