Antibodies:

5H8/4C1

Sensitivity:

0.5ng/ml

Limit of Detection:

2ng/ml

Assay Performance:

Intra-assay CV% of 6.4; Inter-assay CV% of 15

Contents:

Monoclonal antibody 5H8, Universal   Allergen Standard, Biotinylated antibody 4C1; sufficient reagents for 10   plates.

Storage:

Store at 4°C.

Not Provided:

50mM Carbonate/Bicarbonate buffer, pH 9.6

  Phosphate Buffered Saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)

  Bovine Serum Albumin

  Streptavidin-Peroxidase

  1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:

Product Resources:

EL-DP1 Certificate of Analysis

For research and commercial use in vitro: not for   human in vivo or therapeutic use.

Certificate of Analysis

Monoclonal Antibody:

5H8 (clone 5H8 C12 D8)

Vial:

Red Cap; 100μl

Immunogen:

Der p 1

Isotype:

Mouse IgG2A

Specificity:

Binds to an epitope present on Dermatophagoides   pteronyssinusallergen, Der p 1.

Purification:

Produced in tissue culture and purified by affinity   chromatography using Protein A. Single heavy and light chain bands on   SDS-PAGE.

Concentration:

2.0mg/ml in phosphate buffered saline, pH 7.4.    Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative   free.

Monoclonal Antibody:

4C1 (clone 4C1 B8 3F8)

Vial:

Brown Cap; 100μl

Immunogen:

Der f 1

Isotype:

Mouse IgG1

Specificity:

Binds to an epitope on dust mite Dermatophagoides Group   1 allergens (Der f 1, Der p 1, Der m 1, Eur m 1).

Purification:

Produced in ascites and purified by affinity   chromatography using Protein A. Single heavy and light chain bands on   SDS-PAGE.

Biotinylation:

Biotinylated and titrated for use in ELISA at 1/1000   dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered,   preservative free.

Allergen Standard:

Universal Allergen Standard

Vial:

White Cap; 400μl

Composition:

A formulation of eight purified natural allergens   prepared in 1% BSA/50% glycerol/PBS, pH 7.4.

Concentration/Calibration:

Universal Allergen Standard

Protein Measurement

Concentration (ng/ml)

Der p 1

Amino-acid analysis

2500

Der f 1

Amino-acid analysis

2500

Der p 2

Amino-acid analysis

1000

Fel d 1

Amino-acid analysis

1000

Can f 1

Amino-acid analysis

2500

Rat n 1

Amino-acid analysis

1000

Mus m 1

Amino-acid analysis

250

Bla g 2

Amino-acid analysis

2500

References:

1. Luczynska CM, Arruda LK, Platts-Mills TA,   Miller JD, Lopez M, Chapman MD. A two-site monoclonal antibody ELISA for the   quantification of the major Dermatophagoides spp. allergens, Der p I and Der   f I. J Immunol Methods 1989; 118(2):227-235.

2. Custovic A, Taggart SC, Francis HC, Chapman MD,   Woodcock A. Exposure to house dust mite allergens and the clinical activity   of asthma. J Allergy Clin Immunol 1996; 98(1):64-72.

3. Platts-Mills TA, Vervloet D, Thomas WR, Aalberse RC,   Chapman MD. Indoor allergens and asthma: report of the Third International   Workshop. J Allergy Clin Immunol 1997; 100(6 Pt 1):S2-24.

4. Sawyer G, Kemp T, Shaw R et al. Biologic pollution   in infant bedding in New Zealand: high allergen exposure during a vulnerable   period. J Allergy Clin Immunol 1998; 102(5):765-770.

5. Arbes SJ, Jr., Cohn RD, Yin M et al. House dust   mite allergen in US beds: results from the First National Survey of Lead and   Allergens in Housing. J Allergy Clin Immunol 2003; 111(2):408-414.

6. Gruchalla RS, Pongracic J, Plaut M et al. Inner City   Asthma Study: relationships among sensitivity, allergen exposure, and asthma   morbidity. J Allergy Clin Immunol 2005; 115(3):478-485.

7. Zock JP, Heinrich J, Jarvis D et al. Distribution   and determinants of house dust mite allergens in Europe: the European   Community Respiratory Health Survey II. J Allergy Clin Immunol 2006;   118(3):682-690.

8. Jarvis D, Zock JP, Heinrich J et al. Cat and dust   mite allergen levels, specific IgG and IgG(4), and respiratory symptoms in   adults. J Allergy Clin Immunol 2007; 119:697-704.

9. Earle CD, King EM, Tsay A et al. High-throughput   fluorescent multiplex array for indoor allergen exposure assessment. J   Allergy Clin Immunol 2007; 119(2):428-433.

(For complete reference list see EL-DP1 Certificate of Analysis)